HPLC Separation of β-Lactam Antibiotics such as Amoxicillin and Cephalexin on Primesep 100 Column

HPLC Method for Cephalexin, Amoxicillin, Amoxicillin hydrate (1:3) on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cephalexin, Amoxicillin, Amoxicillin hydrate (1:3).

Both amoxicillin and cephalexin are beta-lactam antibiotics. Beta-lactam antibiotics contain a beta-lactam ring in their molecular structure. As a group, these drugs are active against many gram-positive, gram-negative and anaerobic organisms. Amoxicillin is a prescription antibiotic with the molecular formula C16H19N3O5S. It is typically used to treat bacterial infections and is taken orally.  Cephalexin has the chemical formula C16H17N3O4S.

Cephalexin, Amoxicillin, Amoxicillin hydrate (1:3) can be retained and baseline separated with an isocratic method in 30/70 Acetonitrile (ACN) and water mobile phase with a Sulfuric acid (H2SO4) buffer. UV detection at 250 nm.

Condition
Column Primesep 100, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O
Buffer TFA, H2SO4
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description
Class of Compounds
 Drug, Antibiotics, Hydrophobic, Ionizable
Analyzing Compounds Cephalexin, Amoxicillin, Amoxicillin hydrate (1:3)

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Amoxicillin
Amoxicillin hydrate (1:3)
Cephalexin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.