Amoxicillin hydrate (1:3)

Amoxicillin hydrate (1:3) structural formula

CAS Number61336-70-7
Molecular FormulaC16H25N3O8S
Molecular Weight419.450
InChI KeyMQXQVCLAUDMCEF-CWLIKTDRSA-N
LogP0.601
Synonyms
  • Amoxicillin hydrate (1:3)
  • (2S,5R,6R)-6-{[(2R)-2-Amino-2-(4-hydroxyphenyl)acetyl]amino}-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid--water (1/3)
  • 4-Thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid, 6-[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino]-3,3-dimethyl-7-oxo-, (2S,5R,6R)-, hydrate (1:3)
  • 61336-70-7
  • Alfoxil
  • alpha-Amino-p-hydroxybenzylpenicillin trihydrate
  • Amoxicillin trihydrate
  • Amoksicillin forte
  • Amoxi-wolff
  • Amoxicillin-ratiopharm
  • Benzoral
  • Dura AX
  • Uro-clamoxyl
  • Paradroxil
  • Metifarma
  • Kentrocyllin
  • Amophar
  • Amoxi-Diolan
  • Amoxillat
  • Amoxina
  • Amoxine
  • Flemoxine
  • Galenamox
  • Himinomax
  • Pacetocin
  • Siganopen
  • Sintopen
  • Zamocilline
  • UNII-804826J2HU

Applications:

HPLC Separation of β-Lactam Antibiotics such as Amoxicillin and Cephalexin on Primesep 100 Column

March 18, 2021

Separation type: Liquid Chromatography Mixed-mode


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High Performance Liquid Chromatography (HPLC) Method for Analysis of Amoxicillin and Cephalexin.

Both amoxicillin and cephalexin are beta-lactam antibiotics. Beta-lactam antibiotics contain a beta-lactam ring in their molecular structure. As a group, these drugs are active against many gram-positive, gram-negative and anaerobic organisms. Both compounds can be retained and baseline separated with an isocratic method in 30/70 Acetonitrile (ACN) and water mobile phase with a Sulfuric acid (H2SO4) buffer. UV detection at 250 nm.

Condition
Column Primesep 100, 4.6×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA, H2SO4
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description
Class of Compounds
 Drug, Antibiotics, Hydrophobic, Ionizable
Analyzing Compounds Cephalexin,  Amoxicillin

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Amoxicillin
Amoxicillin hydrate (1:3)
Cephalexin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Amoxicillin hydrate (1:3) on Newcrom R1 HPLC column

February 16, 2018
Separation of Amoxicillin hydrate (1:3) on Newcrom C18 HPLC column

Amoxicillin hydrate (1:3) can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Amoxicillin hydrate (1:3)
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.