HPLC Separation of β-Lactam Antibiotics such as Amoxicillin and Cephalexin on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode


HPLC.cloud

View on hplc.cloud

High Performance Liquid Chromatography (HPLC) Method for Analysis of Amoxicillin and Cephalexin.

Both amoxicillin and cephalexin are beta-lactam antibiotics. Beta-lactam antibiotics contain a beta-lactam ring in their molecular structure. As a group, these drugs are active against many gram-positive, gram-negative and anaerobic organisms. Both compounds can be retained and baseline separated with an isocratic method in 30/70 Acetonitrile (ACN) and water mobile phase with a Sulfuric acid (H2SO4) buffer. UV detection at 250 nm.

Condition
Column Primesep 100, 4.6×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA, H2SO4
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description
Class of Compounds
 Drug, Antibiotics, Hydrophobic, Ionizable
Analyzing Compounds Cephalexin,  Amoxicillin

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Amoxicillin
Amoxicillin hydrate (1:3)
Cephalexin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.