HPLC Method for Analysis of 4-Nitrobenzoyl Chloride on Primesep 200 Column

HPLC Method for 4-Nitrobenzoyl chloride on Primesep 200 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Epibatidine

4-nitrobenzoyl chloride is an acyl halide with the chemical formula C7H4ClNO3. It is primarily used in preparation of polysubstituted furanonaphthoquinoines and as an intermediate in active pharmaceutical ingredients and dyes. You can find detailed UV spectra of 4-Nitrobenzoyl chloride and information about its various lambda maxima by visiting the following link.

4-Nitrobenzoyl chloride can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

ColumnPrimesep 200, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 40/60%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/m
DetectionUV 200 nm
Class of Compounds
 Acyl Halide
Analyzing Compounds4-Nitrobenzoyl chloride

Application Column

Primesep 200

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
4-Nitrobenzoyl chloride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.