HPLC MS Method for Analysis of Streptomycin on Chromni Column

HPLC Method for Analysis of Streptomycin on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Streptomycin.

Streptomycin is the an antibiotic with the chemical formula C21H39N7O12. It is primarily used to treat bacterial infections but not limited to tuberculosis, endocarditis, brucellosis, and the plague. It is typically given intramuscularly. It works as a protein synthesis inhibitor by binding to the 16S rRNA of the 30S ribosomal subunit, which leads to it interfering with the binding of formyl-methionyl-tRNA. It is the official state microbe of New Jersey. You can find detailed UV spectra of Streptomycin and information about its various lambda maxima by visiting the following link.

Streptomycin can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using LC MS.

Condition

ColumnChromni, 3.2 x 100 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O
BufferAmmonium Formate
Flow Rate0.5 ml/min
DetectionLC MS ESI SIM 582 (M+H)+
LOD*0.2 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsAntibiotic
Analyzing CompoundsStreptomycin

Application Column

Chromni

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Streptomycin
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.