HPLC Method for Analysis of Acetyl-coenzyme A (acetyl-CoA) on Chromni Column

HPLC Method for Analysis of Acetyl-coenzyme A on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Acetyl-coenzyme A.

Acetyl-coenzyme A, also known as Acetyl-CoA, is the a molecule with the chemical formula C23H38N7O17P3S. It mainly participates in biochemical reactions responsible for protein, carbohydrate, and lipid metabolism. It’s main function is to deliver the acetyl group to the Krebs cycle for energy production. The discovery of that linked this molecule to metabolism was awarded a Nobel Prize in Physiology or Medicine.

Acetyl-coenzyme A can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using LC MS.

Condition

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated
Mobile PhaseMeCN/H2O
BufferAmAc pH 5.0 – 20 mM
Flow Rate1.0 ml/min
Detection260 UV nm
LOD*27.7 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsMetabolic Intermediate
Analyzing CompoundsAcetyl-coenzyme A

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

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Application Analytes:
Acetyl-coenzyme A
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.