HPLC Method for Determination of Vecuronium on Primesep SB Column

HPLC Method for Vecuronium bromide on Primesep SB by SIELC Technologies

HPLC Method for Determination of Vecuronium on Primesep SB Column

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Vecuronium bromide

Vecuronium bromide, also known as Norcuron, is a muscle relaxant with the chemical formula C34H57BrN2O4. It is primarily used during surgery and executions by lethal injection. Reversal of vecuronium’s effects can be achieved through administration sugammadex.

HPLC methods for Vecuronium bromide are of great interest to pharmaceutical research and industry. Vecuronium bromide can be separated and retained on a mixed-mode stationary phase Primesep SB column with embedded strong basic ion-pairing groups, using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) ionic modifier. This analysis method can be UV detected at 260 nm with high resolution and peak symmetry and is compatible with Mass Spectrometry (MS), ELSD, and CAD.

Condition
Column Primesep SB, 2.1 x 100 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 10%
Buffer Ammonium Formate pH 3.0 – 50 mM, 100 mM
Flow Rate 0.2 ml/min
Detection ELSD, 50C

 

Description
Class of Compounds Drug
Analyzing Compounds Vecuronium bromide

Application Column

Primesep SB

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Vecuronium bromide
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.