HPLC Method for Lysine on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Lysine.
Lysine is an essential amino acid with the chemical formula C6H14N2O2. It’s primary role is in proteinogenesis, but it also plays a part in uptake of essential mineral nutrients, production of carnitine, and histone modification. Lysine is known in pop culture thanks to Jurassic Park, in which dinosaurs were genetically altered to be unable to produce lysine. While in the story, this made the dinosaurs be unable to survive in the wild due to needing the lysine supplements provided by the staff at Jurassic Park, no real animal can produce lysine naturally. Lysine is another essential amino acid typically found in meat, and also cereal grains. You can find detailed UV spectra of Lysine and information about its various lambda maxima by visiting the following link.
Lysine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) buffer. Detection is performed using UV.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | H2SO4 – 0.2% |
| Buffer | MeCN/H2O – 10/90% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200 nm |
| LOD* | 15 ppm |
| Class of Compounds | Amino Acid |
| Analyzing Compounds | Lysine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
