HPLC Method for Lorazepam Injection on Newcrom R1 Column

HPLC Method for Lorazepam, Benzyl alcohol on Newcrom R1 by SIELC Technologies

 

 

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Lorazepam.

Lorazepam is a short-acting benzodiazepine medication commonly used as a sedative and anxiolytic of choice in the inpatient setting owing to its fast onset of action when administered intravenously. Lorazepam is one of the few sedative-hypnotics with a relatively clean side effect profile. It has the chemical formula C15H10Cl2N2O2.

You can find detailed UV spectra of Lorazepam, Benzyl alcohol and information about its various lambda maxima by visiting the following link.

Lorazepam can be retained in HPLC on Newcrom R1 reverse-phase column with the simple isocratic mobile phase consisting of acetonitrile (MeCN) and water. The analysis method can be UV detected at 275 nm.

Condition
Column Newcrom R1, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 35%
Buffer No
Flow Rate 1.0 ml/min
Detection UV 275 nm

Description
Class of Compounds Hydrophobic, Drug
Analyzing Compounds Lorazepam, Benzyl alcohol

 

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Benzyl alcohol
Lorazepam
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.