High Performance Liquid Chromatography (HPLC) Method for Analysis of Phenformin on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Phenformin
Biguanides, including Moroxydine, Metformin, Proguanil, and Phenformin, are a group of organic compounds that share a common structural feature, namely the presence of two guanidine groups.
Phenformin is an antidiabetic biguanide that was previously used to treat type 2 diabetes. However, due to the risk of lactic acidosis (a serious side effect), it has been largely withdrawn from the market in many countries.
These biguanides exhibit various pharmacological activities, and while some are primarily used for antidiabetic purposes, others have applications in antiviral or antimalarial treatments. It’s important to note that the safety and efficacy of these drugs depend on proper medical supervision and adherence to prescribed dosages.
Phenformin can be retained and analyzed using a Primesep 100 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV at 240 nm
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O 75/25% |
| Buffer | AmFm pH 3.0 – 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 240 nm |
| Samples | 0.5 mg/ml |
| Injection volume | 2 µl |
| LOD* | 13 ppb |
| Class of Compounds | Biguanides |
| Analyzing Compounds | Phenformin |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
