HPLC Method For Analysis Of Trimetazidine Hydrochloride on Primesep 100 Column

HPLC Method for Trimetazidine on Primesep 100 by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Trimetazidine.

Trimetazidine (TMZ) is a medication with the chemical formula C14H22N2O3. It is taken to treat angina and other heart conditions. It inhibits the mitochondrial enzyme long-chain-3-ketoacyl-CoA thiolase  which decreases fatty acids and shifts substrate utilization towards glucose oxidation. There is a debate within the sports and scientific communities about whether or not TMZ is a performance-enhancing drug. The World Anti-Doping Agency considers it to be a hormone and metabolic modulator.

Trimetazidine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Condition
Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 60/40%
Buffer H2SO4 – 0.4%
Flow Rate 1.0 ml/min
Detection UV, 200 nm,

 

Description
Class of Compounds
 Drug
Analyzing Compounds Trimetazidine

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Trimetazidine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.