Trimetazidine structural formula

CAS Number5011-34-7
Molecular FormulaC14H22N2O3
Molecular Weight266.342
  • Trimetazidine
  • 1-[(2,3,4-Trimethoxyphenyl)methyl]piperazine
  • Piperazine, 1-[(2,3,4-trimethoxyphenyl)methyl]-
  • 5011-34-7
  • EINECS 225-690-2
  • Trimetazidina
  • Trimetazidinum
  • UNII-N9A0A0R9S8


HPLC Method For Analysis Of Trimetazidine Hydrochloride on Primesep 100 Column

March 22, 2022

Separation type: Liquid Chromatography Mixed-mode
View on


High Performance Liquid Chromatography (HPLC) Method for Analysis of Trimetazidine Hydrochloride
λmax  270 nm

Trimetazidine (TMZ) is a popular medication taken to treat angina and other heart conditions. It works by breaking down fatty acids and allowing for the heart to be better oxygenated. TMZ can be retained and analyzed on a mixed-mode Primesep 100 column with a mobile phase consisting of water, Acetonitrile (MeCN), and Perchloric acid (HClO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.



Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 60/40%
Buffer H2SO4 – 0.4%
Flow Rate 1.0 ml/min
Detection UV, 200 nm,



Class of Compounds
Analyzing Compounds Trimetazidine Hydrochloride


Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.

Separation of Trimetazidine on Newcrom R1 HPLC column

February 16, 2018
Separation of Trimetazidine on Newcrom C18 HPLC column

Trimetazidine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: or by phone: 847-229-2629.