HPLC Method for Analysis of Sucralose, Mannitol, and Lactulose on Primesep S2 Column Using Mass Spectrometry

HPLC Method for Sucralose, Mannitol, Lactulose on Primesep S2 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Sucralose, Mannitol, Lactulose

Sucralose is an synthetic organochlorine compound with the molecular formula C12H19Cl3O8.

Properties:
Appearance: Typically a white, odorless, crystalline powder.

Molecular weight: ~397.6 g/mol

Solubility: Soluble in water.

Uses: An artificial sweetener used to provide sweetness in food and beverages without the calories of sugar. 

Mannitol is an organic compound with the molecular formula C6H14O6.

Properties:
Appearance: Typically a white, odorless, crystalline powder or granules.

Molecular weight: ~182.17 g/mol

Solubility: Soluble in water.

Uses: A versatile medication with various uses in healthcare, primarily as an osmotic diuretic. 

Lactulose is an synthetic disaccharide with the molecular formula C12H22O11.

Properties:
Appearance: Typically a white, odorless crystalline powder with a sweet taste.

Molecular weight: ~342.30 g/mol

Solubility: Soluble in water.

Uses: Used for treating constipation and hepatic encephalopathy (HE). 

Sucralose, Mannitol, Lactulose can be retained and analyzed using the Primesep S2 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and formic acid acid. Detection is performed using MS.

ColumnPrimesep S2, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 85%
BufferFormic Acid – 0.2%
Flow Rate0.5 ml/min
DetectionMS ESI –

Class of CompoundsSugar
Analyzing CompoundsSucralose, Mannitol, Lactulose

Application Column

Primesep S2

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Lactulose
Mannitol
Sucralose
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.