HPLC Method for Analysis of Selenous Acid on Chromni Column

HPLC Method for Analysis of Selenious Acid on Chromni Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Selenious Acid.

Selenous Acid, also known as selenious acid, is a compound with the formula H2SeO3. It is used primarily to change the color of steel when used in a process with copper (II) nitrate and nitric acid. The process, known as cold-bluing, causes steel to change from a grey to blue-grey or even black. It can be used to darken other metals as well. You can find detailed UV spectra of Selenious Acid and information about its various lambda maxima by visiting the following link.

Selenious Acid can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using ELSD.

Condition

ColumnChromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated
Mobile PhaseMeCN/H2O – 80/20
BufferAmAc pH 3.0 – 10 mM
Flow Rate1.0 ml/min
DetectionELSD, the nebulizer and evaporator temperatures
50°C with a gas flow rate of 1.6 Standard Liters per Minute (SLM)
LOD*0.5 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsAcid
Analyzing CompoundsSelenious Acid

Application Column

Chromni

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated

Add to cart
Application Analytes:
Selenious Acid
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.