| CAS Number | 7783-00-8 |
|---|---|
| Molecular Formula | H2SeO3 or H2O3Se |
| Molecular Weight | 128.99 |
| InChI Key | MCAHWIHFGHIESP-UHFFFAOYSA-N |
| Synonyms |
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Applications:
HPLC Method for Analysis of Selenous Acid on Chromni Column
October 2, 2026
HPLC Method for Analysis of Selenious Acid on Chromni Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Selenious Acid.
Selenous Acid, also known as selenious acid, is a compound with the formula H2SeO3. It is used primarily to change the color of steel when used in a process with copper (II) nitrate and nitric acid. The process, known as cold-bluing, causes steel to change from a grey to blue-grey or even black. It can be used to darken other metals as well. You can find detailed UV spectra of Selenious Acid and information about its various lambda maxima by visiting the following link.
Selenious Acid can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using ELSD.
Condition
| Column | Chromni, 4.6 x 150 mm, 3 µm, 100 A, surface coated |
| Mobile Phase | MeCN/H2O – 80/20 |
| Buffer | AmAc pH 3.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| LOD* | 0.5 ppm |
Description
| Class of Compounds | Acid |
| Analyzing Compounds | Selenious Acid |
Application Column
Chromni
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: surface coated
Uv-Vis Spectrum of Selenous Acid
March 3, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Selenious Acid check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
HPLC Method for Analysis of Selenious Acid (Se 4 + form) on Newcrom BH Column
July 11, 2017
Selenious Acid is used in protecting and changing the color of steel, most often in weaponry. It is highly toxic in excessive quantities, and if ingested, it could lead to death. The Acid is also capable of penetrating the skin and can produce acute poisonings. Newcrom BH, a reverse-phase column, contains embedded basic ionizable groups and can retain Selenious Acid. The method is UV compatible and can be used as a general approach for analyzing similar compounds.
| Column | Newcrom BH, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H3PO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Selenious Acid |
Application Column
Newcrom BH
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
