HPLC Method for Analysis of Pyrilamine on Newcrom AH Column

HPLC Method for Pyrilamine on Newcrom AH by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Pyrilamine

Pyrilamine, also known as mepyramine, is a first-generation antihistamine with the molecular formula C17H23N3O. It works through targeting the H1 receptor and blocking effects of histamines. It can relieve allergy symptoms like sneezing, runny nose, and itchy, watery eyes. Due to it’s ability to cross the blood-brain carrier, it might have a strong drowsiness side-effect after ingestion. When used topically, it is used to treat skin reactions to bug bites, stings, nettles rashes, and more.

Pyrilamine can be retained and analyzed using the Newcrom AH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV 310nm and LC MS ESI SIM 286.

You can find detailed UV spectra of Pyrilamine and information about its various lambda maxima by visiting the following link.

ColumnNewcrom AH, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferAmmonium Formate pH 3.0
Flow Rate1.0 ml/min
DetectionUV 222 / LC MS ESI SIM 286 [M+H] +
LOD* UV35 ppb
LOD* LC MS ESI SIM0.5 ppm
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsAntihistamine
Analyzing CompoundsPyrilamine

Application Column

Newcrom AH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Pyrilamine
Application Detection:
UV Detection
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.