HPLC Method for Analysis of Pseudoephedrine and Fexofenadine from Allegra D Tablet on Primesep 100 Column

Separation type: Liquid Chromatography Mixed-mode

 

HPLC Method for Analysis of Pseudoephedrine and Fexofenadine from Allegra D Tablet on Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Pseudoephedrine and Fexofenadine

Pseudoephedrine is one of the most popular decongestant drugs on the market (in part due to its efficacy and in part due to its status as an amphetamine). Fexofenadine is a common antihistamine used to treat hives, itching, and hay fever. Pseudoephedrine and Fexofenadine can be separated, retained, and analyzed on a Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 200 nm with high resolution and peak symmetry.

Condition
Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 65%
Buffer H2SO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV 200 nm

 

Description
Class of Compounds
 Drug
Analyzing Compounds Pseudoephedrine,  Fexofenadine

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Fexofenadine hydrochloride
Pseudoephedrine (PSE)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.