| CAS Number | 153439-40-8 |
|---|---|
| Molecular Formula | C32H40ClNO4 |
| Molecular Weight | 538.130 |
| InChI Key | RRJFVPUCXDGFJB-UHFFFAOYSA-N |
| LogP | 4.14 |
| Synonyms |
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Applications:
HPLC Method for Analysis of Pseudoephedrine and Fexofenadine from Allegra D Tablet on Primesep 100 Column
July 22, 2022
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Pseudoephedrine and Fexofenadine
Pseudoephedrine is one of the most popular decongestant drugs on the market (in part due to its efficacy and in part due to its status as an amphetamine). Fexofenadine is a common antihistamine used to treat hives, itching, and hay fever. Pseudoephedrine and Fexofenadine can be separated, retained, and analyzed on a Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 200 nm with high resolution and peak symmetry.
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 65% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm |
| Class of Compounds |
Drug |
| Analyzing Compounds | Pseudoephedrine, Fexofenadine |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsPseudoephedrine (PSE)
Separation of Fexofenadine hydrochloride on Newcrom R1 HPLC column
February 16, 2018
Fexofenadine hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options