HPLC Method for Analysis of Prednisolone on Primesep 100 Column on Alltesta™

HPLC Method for Prednisolone on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Prednisolone

Prednisolone is a steroid hormone with the molecular formula C21H28O5. It is a synthetic version of natural hormones as well as a corticosteroid medication and is used to reduce inflammation and suppress the immune system. Some of the conditions that it treats include: allergic reactions, autoimmune diseases. respiratory illnesses, skin conditions, and certain blood disorders.

Prednisolone can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 275 nm

Class of CompoundsSynthetic Hormone
Analyzing CompoundsPrednisolone

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Prednisolone
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.