HPLC Method for Nitroglycerin on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Nitroglycerin
Nitroglycerin is a chemical compound with C3H5N3O9 as it’s molecular formula. Historically, Nitroglycerin has been used as an explosive. These days, however, it is more commonly used as a vasodilator medication through widening blood vessels.
Nitroglycerin can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
Column | Newcrom R1, 4.6 x 100 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeCN/H2O – 50/50% |
Buffer | H3PO4 – 0.1 % |
Flow Rate | 1.0 ml/min |
Detection | UV, 215 nm |
Class of Compounds | Drug, Basic, Hydrophobic, Ionizable, Vasodilator |
Analyzing Compounds | Nitroglycerin |
Application Column
Newcrom R1
Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended