HPLC Method for Analysis of Nitroglycerin on Newcrom R1 Column

HPLC Method for Nitroglycerin on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Nitroglycerin 

Nitroglycerin is a chemical compound with C3H5N3O9 as it’s molecular formula. Historically, Nitroglycerin has been used as an explosive. These days, however, it is more commonly used as a vasodilator medication through widening blood vessels.

Nitroglycerin can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.

ColumnNewcrom R1, 4.6 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 50/50%
BufferH3PO4 – 0.1 %
Flow Rate1.0 ml/min
DetectionUV, 215 nm
Class of Compounds
Drug, Basic, Hydrophobic, Ionizable, Vasodilator
Analyzing CompoundsNitroglycerin

Application Column

Newcrom R1

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Nitroglycerin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.