HPLC Method for Analysis of Myelin Basic Protein (85-99) Peptide Antagonist on BIST B+ Column

HPLC Method for Myelin Basic Protein Peptide Antagonist on BIST B+ by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Myelin Basic Protein Peptide Antagonist

Myelin Basic Protein Peptide Antagonist is a synthetic peptide antagonist with the molecular formula C70H114N18O21 • XCF3COOH. When expressing an MBP (85-99), it inhibits the production of IL-2 in T cell hybridoma cells. MBP peptide antagonist also delays MBP (85-99)-induced experimental autoimmune encephalomyelitis and reduces symptom severity.

Myelin Basic Protein Peptide Antagonist can be retained and analyzed using the BIST B+ stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.

ColumnBIST B+, 4.6 x 50 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeCN – 70/30% – 50/50%, 10 min
BufferSulfuric Acid
Flow Rate1.0 ml/min
DetectionUV 200
LOD* UV194 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsPeptide Antagonist
Analyzing CompoundsMyelin Basic Protein Peptide Antagonist

Application Column

BIST B+

Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Myelin Basic Protein Peptide Antagonist
Application Detection:
UV Detection
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