| CAS Number | 444305-16-2 |
|---|---|
| Molecular Formula | C70H114N18O21 • XCF3COOH |
| Molecular Weight | 1543.8 |
| InChI Key | JHPGEXWNFDTTLX-RMOYKTNBSA-N |
| Synonyms |
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Applications:
HPLC Method for Analysis of Myelin Basic Protein (85-99) Peptide Antagonist on BIST B+ Column
November 4, 2025
HPLC Method for Myelin Basic Protein Peptide Antagonist on BIST B+ by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Myelin Basic Protein Peptide Antagonist
Myelin Basic Protein Peptide Antagonist is a synthetic peptide antagonist with the molecular formula C70H114N18O21 • XCF3COOH. When expressing an MBP (85-99), it inhibits the production of IL-2 in T cell hybridoma cells. MBP peptide antagonist also delays MBP (85-99)-induced experimental autoimmune encephalomyelitis and reduces symptom severity.
Myelin Basic Protein Peptide Antagonist can be retained and analyzed using the BIST B+ stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV.
| Column | BIST B+, 4.6 x 50 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 70/30% – 50/50%, 10 min |
| Buffer | Sulfuric Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 |
| LOD* UV | 194 ppb |
| Class of Compounds | Peptide Antagonist |
| Analyzing Compounds | Myelin Basic Protein Peptide Antagonist |
Application Column
BIST B+
Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
UV-Vis Spectrum of Myelin Basic Protein (85-99) peptide antagonist
November 4, 2025
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.
