HPLC Method for Levothyroxine (T4) on Primesep D by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Levothyroxine (T4)
Levothyroxine Sodium is a synthetic thyroid hormone that is used to treat hypothyroidism by replacing T4 (Levothyroxine) that is not being produced in necessary accounts. IT’s absorption can be affected by antacids, calcium, and iron salts.
Levothyroxine Sodium can be retained and analyzed using the Primesep D stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a trifluoroacetic acid buffer. Detection is performed using UV.
| Column | Primesep D, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | TFA – 0.1 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 230 nm |
| Class of Compounds |
Drug, Hormone, Acid, Hydrophobic |
| Analyzing Compounds | Levothyroxine (T4) |
Application Column
Primesep D
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Levothyroxine (T4)Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
