HPLC UV Method for Analysis of Imetit  on Primesep A  Column

HPLC Method for Analysis of [compound] on Primesep A Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Imetit.

Imetit is a histamine H3 receptor agonist with the chemical formula C6H10N4S. You can find detailed UV spectra of Imetit and information about its various lambda maxima by visiting the following link.

Imetit can be retained and analyzed using the Primesep A stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a [buffer] buffer. Detection is performed using UV.

Condition

ColumnPrimesep A, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 80%
BufferH2SO4 – 0.4%
Flow Rate1.0 ml/min
DetectionUV 200
Limit Of Detection*130 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Description

Class of CompoundsHistamine Receptor Agonist
Analyzing CompoundsImetit

Application Column

Primesep A

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Imetit
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.