HPLC Method For Analysis of Erythrosine on Newcrom BH Column

HPLC Method for Erythrosine on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Erythrosine

Erythrosine (also known as Red #3) is a fluorescent red acid dye used for food coloring, pharmaceutical dye, in printing inks, as a biological stain, and as a dental plaque disclosing agent. It is banned in the european union for health risks. It has the chemical formula C20H6I4Na2O5.

Erythrosine can be retained on HPLC mixed-mode Newcrom BH column using a mobile phase consisting of methanol (MeOH) and water with perchloric acid (HClO4) buffer. The analysis method can be UV detected at 240 or 498 nm.

Condition
Column Newcrom BH, 2.1 x 100 mm, 5 µm, 100 A, dual ended
Mobile Phase MeOH/H2O – 75/25%
Buffer HClO4 – 0.5%
Flow Rate 0.2 ml/min
Detection UV 240, 480  nm

Description
Class of Compounds Acid, Dyes
Analyzing Compounds Erythrosine

 

Application Column

Newcrom BH

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Erythrosine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.