HPLC Method for Analysis of Econazole on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Econazole
Econazole is an antifungal medication primarily used to treat a variety of fungal skin infections. It belongs to the imidazole class of antifungal agents. Here are some key points about econazole:
Mechanism of Action:
Like other imidazole antifungals, econazole works by inhibiting the enzyme responsible for producing ergosterol, a key component of the fungal cell membrane. A lack of ergosterol disrupts the integrity of the fungal cell membrane, leading to the death of the fungus.
Econazole can be retained, and analyzed on a Primesep 200 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid as a buffer. This analysis method can be detected in the UV 200 nm.
| Column | Primesep 200, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | Gradient MeCN – 40-70%, 10 min |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm |
| Peak Retention Time | 6.89 min |
| Class of Compounds | Allylamines |
| Analyzing Compounds | Econazole |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
