HPLC Method for Denatonium benzoate on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Denatonium benzoate.
Denatonium benzoate, also known as Denatonium, is the bitterest chemical that is safe to be ingested. It is often used as a bittering agent to antifreeze, detergents, and cosmetics to prevent consumption. Even low concentrations of it can cause am incredibly bitter taste, which has led to it’s occasional use in placebo medication to mimic certain other pills. In France and Italy it is mandatory to add Denatonium to antifreeze. It has the chemical formula C₂₈H₃₄N₂O₃. You can find detailed UV spectra of Denatonium benzoate and information about its various lambda maxima by visiting the following link.
Denatonium benzoate can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an Ammonium Formate buffer. Detection is performed using UV and is compatible with Mass Spectrometry (MS), ELSD, and CAD.
| Column | Primesep SB, 4.6 x 150 mm, 3 µm, 100 A, single ended |
| Mobile Phase | Gradient MeCN |
| Buffer | Ammonium Formate pH 3.0 50 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260 nm, MS-compatible mobile phase |
| Class of Compounds |
Acid, Quaternary amine |
| Analyzing Compounds | Denatonium benzoate |
Application Column
Primesep SB
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: single ended
