HPLC Method for D-alpha-Tocopheryl acetate on Newcrom R1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of D-alpha-Tocopheryl acetate
D-alpha-Tocopheryl acetate is a form of Vitamin E with a molucular formula of C31H52O3. It is also known as alpha-tocopheryl acetate. It is often used in cosmetics, food, and dietary supplements due to its antioxidant properties.
D-alpha-Tocopheryl acetate can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
| Column | Newcrom R1, 4.6 x 50 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 98/2% |
| Buffer | H3PO4 – 0.02 % |
| Flow Rate | 2.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds | Drug, Vitamin, Hydrophobic |
| Analyzing Compounds | D-alpha-Tocopheryl acetate |
Application Column
Newcrom R1
Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
D-alpha-Tocopheryl acetateApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
