HPLC Method for Analysis of Allantoin on Primesep 100 Column

HPLC Method for Allantoin on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Allantoin

Allantoin is found in plants such as wheat sprouts, chamomile, sugar beet, and comfrey. It is used as a moisturizer, which is, as the name suggest, used to moisturize the skin and to treat or prevent skin irritations and dry skin. Primesep 100, a reverse-phase column, contains embedded acidic ionizable groups and can retain Allantoine. The method is LC/MS and UV compatible and can be used as a general approach for analyzing similar compounds.

ColumnPrimesep 100, 4.6 x 150 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 10/90%
BufferTFA- 0.1%
Flow Rate1.0ml/min
DetectionUV, 210 nm
Class of Compounds
Plant-based compound
Analyzing CompoundsAllantoin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Allantoin
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.