HPLC Method for Analysis of Adenine on Primesep 100 Column with Alltesta™

HPLC Method for Adenine on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Adenine

Adenine is a chemical compound with the molecular formula C5H5N5. It is a building block for DNA and RNA as one of the four essential nucleotide bases. It is vital as a part of the main energy-carrying molecule in cells. When in DNA, it pairs with thymine, while in RNA, it pairs with uracil.

Adenine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

ColumnPrimesep 100, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 50%
BufferSulfuric Acid
Flow Rate0.5 ml/min
DetectionUV 275 nm

Class of CompoundsNucleotide Base
Analyzing CompoundsAdenine

Application Column

Primesep 100

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Adenine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.