HPLC Method for Analysis of 6-Aminocaproic Acid in a High Salt Content Matrix on Primesep A Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Aminocaproic acid on Primesep AP by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of 6-Aminocaproic acid on Primesep 100 Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of 6-Aminocaproic acid

6-Aminocaproic acid (6ACA) is a synthetic amino acid that is primarily used as a medication.

6-Aminocaproic acid can be retained, separated and analyzed using a Primesep A mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and perchloric acid as a buffer. This method allows for detection using UV 200 nm.

You can find detailed UV spectra of 6-Aminocaproic acid and information about its various lambda maxima by visiting the following link.

ColumnPrimesep AP, 4.6 x 250 mm, 5 µm, 100 A
Mobile PhaseMeCN – 20%
BufferHClO4 -0.2%
Flow Rate1.0 ml/min
DetectionUV 200 nm
Samples1.0 mg/ml in H2O – 100%
Injection volume10 µl
LOD*20 ppm (200 nm)
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Acid
Analyzing Compounds6-Aminocaproic acid

Application Column

Primesep AP

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
6-Aminocaproic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.