HPLC Method for Melanotan-I, Melanotan-II on Chromni by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Melanotan-I, Melanotan-II
Melanotan-I is a synthetic analogue of a-melanocyte-stimulating hormone (a-MSH) with the chemical formula C78H111N21O19. It is used primarily to provide photoprotection against UV radiation as well as for patients with erythropoietic protoporphyria to increase pain-free light exposure. It is used “off-label” as a synthetic tanner, for which it is not FDA approved.
Melanotan-II is a synthetic analogue of peptide hormone a-a-melanocyte-stimulating hormone (a-MSH) with the chemical formula C50H69N15O9. It is approved by the FDA to treat sun sensitivity, but unlicense Melanotan II is sold as a tanning agent, though health agencies advise against it due to reports of it being a health risk. It works as a non-selective agonist of the melanocortin receptors.
Melanotan-I, Melanotan-II can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an sulfuric acid as a buffer. Detection is performed using LC MS.
| Column | Chromni, 3.2 x 100 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN – 80 – 20%, 10 min |
| Buffer | AmFm pH3.0 – 20mM |
| Flow Rate | 0.5 ml/min |
| Detection | LC MS ESI SIM 513 [(M+2H)/2] 2+ ,824 [(M+2H)/2] 2+ |
| Class of Compounds | Analogue |
| Analyzing Compounds | Melanotan-I, Melanotan-II |
Application Column
Chromni
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Melanotan-II
