HPLC ELSD Method for Analysis of Copper Peptide GHK-Cu on Primesep 200 Column

HPLC Method for Copper peptide (GHK-Cu) on Primesep 200 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Copper peptide (GHK-Cu)

Copper Peptide GHK-Cu is a naturally occurring copper complex with the molecular formula C14H22CuN6O4•C2H4O2. Research shows that the complex facilitates faster wound healing, improved angiognesis, and elevated level of antioxidant enzymes. Often times, it is used in anti-aging cosmetics.

Copper peptide (GHK-Cu) can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using ELSD.

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

ColumnPrimesep 200, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 20%
BufferAmmonium formate
Flow Rate0.5 ml/min
DetectionELSD

Class of CompoundsComplex
Analyzing CompoundsCopper peptide (GHK-Cu)

Application Column

Primesep 200

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Copper peptide (GHK-Cu)
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.