Copper peptide (GHK-Cu)

Molecular FormulaC14H23CuN6O4+
Molecular Weight402.92
InChI KeyNZWIFMYRRCMYMN-UHFFFAOYSA-M
Synonyms
  • Copper peptide (GHK-Cu)
  • BCP32687
  • SY253680
  • Copper peptide;GHK-Cu; GHK copper; CG-copper peptide; [N2-(N-Glycyl-L-histidyl)-L-lysinato(2-)]copper
  • Copper(II) (S)-6-Amino-2-[(S)-2-(2-aminoacetamido)-3-(1H-imidazol-5-yl)propanamido]hexanoate

Applications:

HPLC ELSD Method for Analysis of Copper Peptide GHK-Cu on Primesep 200 Column

September 24, 2025

HPLC Method for Copper peptide (GHK-Cu) on Primesep 200 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Copper peptide (GHK-Cu)

Copper Peptide GHK-Cu is a naturally occurring copper complex with the molecular formula C14H22CuN6O4C2H4O2. Research shows that the complex facilitates faster wound healing, improved angiognesis, and elevated level of antioxidant enzymes. Often times, it is used in anti-aging cosmetics.

Copper peptide (GHK-Cu) can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using ELSD.

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

ColumnPrimesep 200, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 20%
BufferAmmonium formate
Flow Rate0.5 ml/min
DetectionELSD

Class of CompoundsComplex
Analyzing CompoundsCopper peptide (GHK-Cu)

Application Column

Primesep 200

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Copper peptide (GHK-Cu)

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC MS Method for Analysis of Copper Peptide GHK-Cu on Primesep 200 Column

September 23, 2025

HPLC Method for Copper peptide (GHK-Cu) on Primesep 200 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Copper peptide (GHK-Cu)

Copper Peptide GHK-Cu is a naturally occurring copper complex with the molecular formula C14H22CuN6O4C2H4O2. Research shows that the complex facilitates faster wound healing, improved angiognesis, and elevated level of antioxidant enzymes. Often times, it is used in anti-aging cosmetics.

Copper peptide (GHK-Cu) can be retained and analyzed using the Primesep 200 stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using LC MS.

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

ColumnPrimesep 200, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 20%
BufferAmmonium formate
Flow Rate0.5 ml/min
DetectionLC MS ESI GHK (free peptide, [M+H]+) 341

Class of CompoundsComplex
Analyzing CompoundsCopper peptide (GHK-Cu)

Application Column

Primesep 200

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Copper peptide (GHK-Cu)

Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Copper peptide GHK-Cu on BIST B Column

June 2, 2022

Separation type: Bridge Ion Separation Technology, or BIST™


HPLC.cloud
View on hplc.cloud

High Performance Liquid Chromatography (HPLC) Method for Analysis of Copper peptide GHK-Cu

Copper peptide GHK-Cu is a copper complex of glycyl-L-histidyl-lysine and can be naturally found in urine, saliva, and plasma. Research has shown that it may promote wound healing, attract immune cells, stimulate collagen, and act as an antioxidant and anti-inflammatory compound. Using SIELC’s newly introduced BIST™ method, Copper peptide GHK-Cu, which separates in water, can be retained on a positively-charged anion-exchange BIST™ B column. There are two keys to this retention method: 1) a multi-charged, negative buffer, such as Sulfuric acid (H2SO4), which acts as a bridge, linking the positively-charged Copper and peptide to the positively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, Copper peptide GHK-Cu can be separated, retained, and UV detected at 210 nm

Condition

Column BIST B, 4.6×50 mm,  100A
Mobile Phase MeCN – 70%
Buffer H2SO4 – 0.2%
Flow Rate 1.0 ml/min
Detection UV 210 nm

Description

Class of Compounds Peptide, Metal
Analyzing Compounds Copper peptide GHK-Cu

 

Application Column

BIST B

BIST™ columns offer a unique and effective way to achieve separations that were traditionally challenging or even impossible with other HPLC columns. With the use of a special mobile phase, these ion exchange columns provide very strong retention for analytes with the same charge polarity as the stationary phase, unlocking new chromatography applications. What makes BIST™ columns stand out is their proprietary surface chemistry, which results in superior selectivity, resolution, and sensitivity. These columns offer a simple, efficient solution for a variety of analytical challenges, making them an excellent choice for researchers and analysts across many different fields. To learn more about the technology that powers BIST™ columns and to explore related applications, check out https://BIST.LC.

Select options
Application Analytes:
Copper peptide (GHK-Cu)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.