HPLC LC MS and ELSD Method for Analysis of 2-Phosphonobutane-1,2,4-tricarboxylic Acid on Newcrom BH Column

HPLC Method for 2-Phosphonobutane-1,2,4-tricarboxylic acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 2-Phosphonobutane-1,2,4-tricarboxylic acid.

PBTC (2-phosphonobutane-1,2,4-tricarboxylic acid) is one of the most widely used scale inhibitors in the cooling water treatment industry. It works through binding with metal ions, therefore preventing them from forming scale. It is widely used especially due to performing well under harsh conditions like high temperatures and high alkalinity. 

Using a mixed-mode column and a mobile phase consisting of acetonitrile (ACN) and water containing formic acid as a buffer, 2-phosphonobutane-1,2,4-tricarboxylic acid can be effectively retained and detected by LC–MS and ELSD.

ColumnNewcrom BH, 2.1 x 50 mm, 3 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 20/80%
BufferFormic acid- 1.0%
Flow Rate0.2 mL/min
DetectionELSD, ESI-SIM: [M-H]1- 269
Class of Compounds
Acid, Hydrophilic, Ionizable
Analyzing Compounds2-Phosphonobutane-1,2,4-tricarboxylic acid

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Application Column

Newcrom BH

Column Diameter: 2.1 mm
Column Length: 50 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
2-Phosphonobutane-1,2,4-tricarboxylic acid
Application Detection:
ELSD Detection
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.