HPLC Method for Adipic Acid, Succinic Acid, Malonic Acid on Chromni by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Adipic Acid, Succinic Acid, Malonic Acid.
Adipic acid, also known as hexanedioic acid, is a key precursor to nylon, and more than 2.5 billion kilograms are produced every year. In medicinal uses, it has been incorporated into controlled-release formulation as well as the polymeric coating of hydrophilic monolithic systems. It has been used as a flavorant and gelling agent in food. It has the chemical formula C6H10O4. You can find detailed UV spectra of Adipic Acid and information about its various lambda maxima by visiting the following link.
Succinic Acid is a dicarboxylic acid with the chemical formula C4H6O4. It is a precursor of some polyesters as well as a component of some alkyd resins. In food, it is typically used as an acidity regulator and flavoring agent. Sulfonic acids like 1-Pentanesulfonic acid, 1-Heptanesulfonic acid, 1-Decanesulfonic acid, and 1=Dodecanesulfonic acid are typically used in ion chromatography and for organic syntheses. You can find detailed UV spectra of Succinic Acid and information about its various lambda maxima by visiting the following link.
Malonic Acid is an organic acid with the C3H4O4 chemical formula. It has a variety of uses from synthesis to preservatives. When it comes to synthesis, it is often used in industrial means. and especially dyes for natural fibers. Besides industrial use, in laboratory environments, it is used in preparation of tris-maleate, sodium maleate buffers, and maleate salts. You can find detailed UV spectra of Malonic Acid and information about its various lambda maxima by visiting the following link.
Adipic Acid, Succinic Acid, Malonic Acid can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using ELSD.
| Column | Chromni, 4.6 x 150 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 90/10% |
| Buffer | AmFm pH 3.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Class of Compounds | Acid, |
| Analyzing Compounds | Adipic Acid, Succinic Acid, Malonic Acid |
Application Column
Chromni
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Malonic Acid
Succinic Acid
