HPLC Method for Sodium dodecyl sulfate on Primesep B4 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium dodecyl sulfate.
Sodium dodecyl sulfate, or sodium lauryl sulfate, is a surfactant used in cleaning and hygienic products. It has a twelve carbon hydrophobic tail attached to a polar sulfate. Dodecyl sulfate can be retained on Primesep B4 column in under 5 minutes with an MS-compatible mobile phase. Primesep B4 is a short carbon chain reverse-phase (RP) mixed-mode column with embedded basic ion-pairing groups.
| Column | Primesep B4 |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | AmFm pH 3.0 – 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Class of Compounds |
Hydrophobic, Base, Ionizable, Anionic surfactant |
| Analyzing Compounds | Sodium dodecyl sulfate |
Application Column
Primesep B4
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Sodium dodecyl sulfateApplication Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
