HPLC Method for Analysis of Sildenafil Citrate (Viagra) in a Tablet Primesep 100 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil or Viagra on Primesep 100 by SIELC Technologies.

Separation type: Liquid Chromatography Mixed-mode

High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil Citrate on Primesep 100 by SIELC Technologies.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sildenafil or Viagra on Primesep 100 by SIELC Technologies.

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Sildenafil or Viagra

Sildenafil Citrate (also known as Viagra) is one of the most popular medications used to treat erectile dysfunction and pulmonary hypertension. Sildenafil can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 214 nm and 294 nm with high resolution and peak symmetry.

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN – 60%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
Detectionλmax 214, 294 nm
Peak Retention Time5.7 min

Description

Class of CompoundsDrug
Analyzing CompoundsSildenafil or Viagra

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Sildenafil or Viagra
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.