HPLC Determination of PEI complex with Cu(II)

HPLC Method for Polyethylenimine PEI on PEI by SIELC Technologies

HPLC Determination of PEI complex with Cu(II)_1177

High Performance Liquid Chromatography (HPLC) Method for Analysis of Polyethylenimine PEI.

Polyethyleneimine (PEI) is a polymer used in a wide range of applications. It is difficult to analyze in HPLC due to several factors. It’s a mixture of compounds with different lengths and therefore different number of charges. It also lacks a UV chromophore. New PEI column was designed specifically for the analysis of polyethyleneimine by ion-exclusion and size-exclusion mechanisms with copper complex to allow for UV detection. The method uses a mobile phase of acetonitrile (ACN) and water with ammonium formate buffer (AmFm) and UV detection at 285nm.

MEASURING PEI Polyethylenimine in biological samples

Condition
Column PEI
Mobile Phase MeCN/H2O – 40/60%
Buffer AmFM pH 3.0  – 20 mM
Flow Rate 0.5 ml/min
Detection UV, 285 nm

 

Description
Class of Compounds
 Polymer, Basic, Hydrophilic, Ionizable
Analyzing Compounds Polyethylenimine PEI

 

Application Column

PEI

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Polyethylenimine PEI
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.