HPLC Determination of Metalaxyl on Newcrom B Column

HPLC Method for Metalaxyl (UV) on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Metalaxyl (UV).

Metalaxyl, also known as methyl N-(methoxyacetyl)-N-(2,6-xylyl)-DL-alaninate,  is a systemic fungicide with the chemical formula C15H21NO4.  It is used to control Pythium and Phytophthora in vegetables. It has suffered a resistance problem going back to the 1980s.

Metalaxyl (UV) can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.

Condition

Column Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 30/70%
Buffer AmFm pH 3.0 –  20 mM
Flow Rate 1.0 ml/min
Detection UV, 266 nm

Description

Class of Compounds Hydrophobic, Herbicide, Pesticide
Analyzing Compounds Metalaxyl (UV)

Application Column

Newcrom B

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Metalaxyl (UV)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.