HPLC Method for Metalaxyl (UV) on Newcrom B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Metalaxyl (UV).
Metalaxyl, also known as methyl N-(methoxyacetyl)-N-(2,6-xylyl)-DL-alaninate, is a systemic fungicide with the chemical formula C15H21NO4. It is used to control Pythium and Phytophthora in vegetables. It has suffered a resistance problem going back to the 1980s.
Metalaxyl (UV) can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium formate buffer. Detection is performed using UV.
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | AmFm pH 3.0 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 266 nm |
| Class of Compounds | Hydrophobic, Herbicide, Pesticide |
| Analyzing Compounds | Metalaxyl (UV) |
Application Column
Newcrom B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended




