HPLC Method for Glyphosine on Newcrom B by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Glyphosine
Glyphosine is a synthetic plant growth regulator with the chemical formula of C4H11NO8P2. It is a colorless liquid that works through decreasing chlorophyll production.
Glyphosine can be retained and analyzed using the Newcrom B stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a formic acid buffer. Detection is performed using CAD.
| Column | Newcrom B, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 10% |
| Buffer | Formic Acid – 3% |
| Flow Rate | 1.0 ml/min |
| Detection | CAD |
| Class of Compounds |
Plant growth regulator, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Glyphosine |
Application Column
Newcrom B
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
GlyphosineApplication Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
