HPLC Method for Darobactin on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Darobactin
The compound was discovered in Photorhabdus bacteria in 2019, living in the digestive systems of entomopathogenic nematodes. Researchers identified the nematode as a possible host because they feed on insects by targeting their larvae and releasing bacteria that then confront pathogens similar to those found in humans.
In experiments, it cured mice of Escherichia coli and Klebsiella pneumoniae infections, without toxic side effects.
| Column | Primesep SB, 2.1 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN |
| Buffer | Ammonium Formate pH 3.0 |
| Flow Rate | 0.2 ml/min |
| Detection | UV 280 nm |
| Class of Compounds | Antibiotic |
| Analyzing Compounds | Darobactin |
Application Column
Primesep SB
Column Diameter: 2.1 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
DarobactinApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
