Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Cromolyn sodium
Cromolyn sodium is a medication used to manage bronchial asthma, allergic rhinitis, and certain allergic eye conditions such as vernal conjunctivitis, keratitis, and keratoconjunctivitis. Cromolyn sodium can be retained in HPLC with a Newcrom BH mixed-mode column using a mobile phase of acetonitrile (ACN) and water and Sulfuric Acid (H2SO4) buffer allowing the use of a UV detector at 240 nm.
Column | Newcrom BH, 4.6×150 mm, 100A |
Mobile Phase | MeCN/H2O – 35/65% |
Buffer | H2SO4 – 0.1% |
Flow Rate | 1.0 ml/min |
Detection | UV 240nm |
Class of Compounds | Drug |
Analyzing Compounds | Cromolyn sodium (Sodium cromoglycate) |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A