HPLC Method for Allantoin on SHARC 1 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Allantoin.
Allantoin is a 5-ureidohydantoin or glyoxyldiureide with the chemical formula C4H6N4O3. It is s metabolic intermediate that is found in plants like chamomile, wheat sprouts, tobacco seed, sugar beet and comfrey. It is frequently used in cosmetics and hygiene products.
Allantoin can be retained using HPLC on SHARC 1 column, which uses hydrogen-bonding as a separation mechanism with isocratic method by using a mobile phase of acetonitrile (ACN) and methanol (MeOH). Allantoin can be monitored by low UV (210 nm), ELSD, CAD or LC/MS.
| Column | SHARC 1, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/MeOH – 90/10% |
| Buffer | No |
| Flow Rate | 1.0 ml/min |
| Detection | UV 210 nm |
| Class of Compounds |
Hydrophilic, Amide, Amine |
| Analyzing Compounds | Allantoin |
Application Column
SHARC 1
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
AllantoinApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
