High Performance Liquid Chromatography (HPLC) Method for Analysis of Alfuzosin on Primesep 100 by SIELC Technologies
Alfuzosin is a medication with the chemical formula C19H27N5O4. It is used to treat the signs and symptoms of benign prostatic hyperplasia (BPH), which is an enlarged prostate gland. It doesn’t shrink the prostate but works by relaxing the muscles of the prostate and the bladder, which helps to relieve symptoms like difficulty in starting the flow of urine, weak stream, and the need to urinate often or urgently. You can find detailed UV spectra of Alfuzosin and information about its various lambda maxima by visiting the following link.
Alfuzosin can be retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid as a buffer. This analysis method can be detected using UV at 254 nm.
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 70/30% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 254 nm |
| Peak Retention Time | 5.86 min |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Alfuzosin |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
