Alfuzosin

Alfuzosin structural formula

CAS Number81403-80-7
Molecular FormulaC19H27N5O4
Molecular Weight389.456
InChI KeyWNMJYKCGWZFFKR-UHFFFAOYSA-N
LogP0.593
Synonyms
  • Alfuzosin
  • N-{3-[(4-Amino-6,7-dimethoxyquinazolin-2-yl)(methyl)amino]propyl}oxolane-2-carboxamide
  • 2-Furancarboxamide, N-[3-[(4-amino-6,7-dimethoxy-2-quinazolinyl)methylamino]propyl]tetrahydro-
  • 81403-80-7
  • 2-Furancarboxamide, N-[3-[(4-amino-6,7-dimethoxy-2-quinazolinyl)methylamino]propyl]tetrahydro-
  • N-(3-((4-Amino-6,7-dimethoxy-2-quinazolinyl)methylamino)propyl)tetrahydro-2-furancarboxamide
  • Alfuzosina
  • Alfuzosine
  • Alfuzosinum
  • UNII-90347YTW5F

Applications:

UV-Vis Spectrum of Alfuzosin

July 19, 2024
UV-Vis Spectrum of Alfuzosin. Absorption Maxima: 212 nm, 246 nm, 332 nm.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Alfuzosin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC – MS Method for Analysis of  Alfuzosin on Primesep 200  Column

September 22, 2023

HPLC Method for Analysis of Alfuzosin on Primesep 200 by SIELC Technologies

HPLC- MS Method for Analysis of  Alfuzosin on Primesep 200  Column  by SIELC Technologies
HPLC- MS Method for Analysis of  Alfuzosin on Primesep 200  Column


 High Performance Liquid Chromatography (HPLC) Method for Analysis of  Alfuzosin

Alfuzosin is a medication used to treat the signs and symptoms of benign prostatic hyperplasia (BPH), which is an enlarged prostate gland. It doesn’t shrink the prostate but works by relaxing the muscles of the prostate and the bladder, which helps to relieve symptoms like difficulty in starting the flow of urine, weak stream, and the need to urinate often or urgently.

Alfuzosin can be retained, and analyzed on a Primesep 200 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format as a buffer. This analysis method can be detected using UV at 247 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)

ColumnPrimesep 200, 2.1 x 100 mm, 5 µm, 100 A
Mobile PhaseMeCN – 70%,
BufferAmmonium Formate pH 3.0-20 mM
Flow Rate0.2 ml/min
DetectionUV, 247 nm, SIM 390 +
Spray Voltage:1.5 kV
Nebulizing gas:1.5 L/min
Drying gas:15 L/min
 DL temp:250 ˚C
Heat Block:400 ˚C

Class of Compounds
Quinazolines, alpha-1 adrenergic receptor antagonist
Analyzing CompoundsAlfuzosin

Application Column

Primesep 200

Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Alfuzosin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Alfuzosin in Tablet Dosage Form on Primesep 100 Column

September 22, 2023

High Performance Liquid Chromatography (HPLC) Method for Analysis of Alfuzosin on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Alfuzosin in Tablet Dosage Form on Primesep 100 Column by SIELC Technologies
HPLC Method for Analysis of Alfuzosin on Primesep 100 Column by SIELC Technologies

Alfuzosin is a medication used to treat the signs and symptoms of benign prostatic hyperplasia (BPH), which is an enlarged prostate gland. It doesn’t shrink the prostate but works by relaxing the muscles of the prostate and the bladder, which helps to relieve symptoms like difficulty in starting the flow of urine, weak stream, and the need to urinate often or urgently.

Alfuzosin can be retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid as a buffer. This analysis method can be detected using UV at 254 nm.

High Performance Liquid Chromatography (HPLC) Method for Analyses of Alfuzosin

Condition

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 70/30%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 254 nm
Peak Retention Time5.86 min

Description

Class of CompoundsDrug
Analyzing CompoundsAlfuzosin

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

Add to cart
Application Analytes:
Alfuzosin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Alfuzosin on Newcrom R1 HPLC column

February 16, 2018
Separation of Alfuzosin on Newcrom C18 HPLC column

Alfuzosin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Alfuzosin
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.