| CAS Number | 81403-80-7 |
|---|---|
| Molecular Formula | C19H27N5O4 |
| Molecular Weight | 389.456 |
| InChI Key | WNMJYKCGWZFFKR-UHFFFAOYSA-N |
| LogP | 0.593 |
| Synonyms |
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Applications:
HPLC – MS Method for Analysis of Alfuzosin on Primesep 200 Column
September 22, 2023
HPLC Method for Analysis of Alfuzosin on Primesep 200 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Alfuzosin
Alfuzosin is a medication used to treat the signs and symptoms of benign prostatic hyperplasia (BPH), which is an enlarged prostate gland. It doesn’t shrink the prostate but works by relaxing the muscles of the prostate and the bladder, which helps to relieve symptoms like difficulty in starting the flow of urine, weak stream, and the need to urinate often or urgently.
Alfuzosin can be retained, and analyzed on a Primesep 200 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a ammonium format as a buffer. This analysis method can be detected using UV at 247 nm, an Evaporative Light Scattering Detector (ELSD), or any other evaporative detection method (CAD, ESI-MS)
| Column | Primesep 200, 2.1 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN – 70%, |
| Buffer | Ammonium Formate pH 3.0-20 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV, 247 nm, SIM 390 + |
| Spray Voltage: | 1.5 kV |
| Nebulizing gas: | 1.5 L/min |
| Drying gas: | 15 L/min |
| DL temp: | 250 ˚C |
| Heat Block: | 400 ˚C |
| Class of Compounds | Quinazolines, alpha-1 adrenergic receptor antagonist |
| Analyzing Compounds | Alfuzosin |
Application Column
Primesep 200
Column Diameter: 2.1 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
HPLC Method for Analysis of Alfuzosin in Tablet Dosage Form on Primesep 100 Column
September 22, 2023
High Performance Liquid Chromatography (HPLC) Method for Analysis of Alfuzosin on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
Alfuzosin is a medication used to treat the signs and symptoms of benign prostatic hyperplasia (BPH), which is an enlarged prostate gland. It doesn’t shrink the prostate but works by relaxing the muscles of the prostate and the bladder, which helps to relieve symptoms like difficulty in starting the flow of urine, weak stream, and the need to urinate often or urgently.
Alfuzosin can be retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid as a buffer. This analysis method can be detected using UV at 254 nm.
High Performance Liquid Chromatography (HPLC) Method for Analyses of Alfuzosin
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 70/30% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 254 nm |
| Peak Retention Time | 5.86 min |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Alfuzosin |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Separation of Alfuzosin on Newcrom R1 HPLC column
February 16, 2018
Alfuzosin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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