HPLC Determination of 6-aminocaproic Acid on Newcrom A Column

HPLC Method for Analysis of 6-Aminocaproic acid on Newcrom A by SIELC Technologies

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6-Aminocaproic acid, brand name Amicar, is a clotting agent used to control excessive bleeding in patients during or after surgery by acting as an inhibitor to fibrinolysis. It can be retained using HPLC and low capacity Newcrom A mixed-mode column using isocratic analysis with mobile phase consisting of acetonitrile and water (ACN/H2O) and MS-compatible ammonium formate (AmFm) buffer.

ColumnNewcrom A, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN/H2O – 10/90%
BufferAmmonium Formate pH 3.0 – 10 мМ
Flow Rate1.0 ml/min
DetectionCAD, Corona (MS-compatible mobile phase)
Class of CompoundsAcid
Analyzing Compounds6-Aminocaproic acid

Application Column

Newcrom A

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
6-Aminocaproic acid
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