HPLC Method for Analysis of 6-Aminocaproic acid on Newcrom A by SIELC Technologies
6-Aminocaproic acid, brand name Amicar, is a clotting agent used to control excessive bleeding in patients during or after surgery by acting as an inhibitor to fibrinolysis. It can be retained using HPLC and low capacity Newcrom A mixed-mode column using isocratic analysis with mobile phase consisting of acetonitrile and water (ACN/H2O) and MS-compatible ammonium formate (AmFm) buffer.
| Column | Newcrom A, 4.6 x 150 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | Ammonium Formate pH 3.0 – 10 мМ |
| Flow Rate | 1.0 ml/min |
| Detection | CAD, Corona (MS-compatible mobile phase) |
| Class of Compounds | Acid |
| Analyzing Compounds | 6-Aminocaproic acid |
Application Column
Newcrom A
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
6-Aminocaproic acid
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