HPLC Determination of Spectinomycin on Obelisc R Column

HPLC Method for Spectinomycin on Obelisc R by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Spectinomycin.

Chloride is typically used to refer to a compound or molecule that contains a chlorine anion: Cl–. It is an electrolyte that is essential for bodily functions including blood pH, fluid balance, cellular functions, and more. Imbalance of chloride can indicate underlying health problems.

Spectinomycin is an antibiotic with the chemical formula C14H24N2O7. It is typically used to treat gonorrhea infections through injection. It works by binding itself to the 30S ribosomal subunit which leads to prevention of movement of mRNA and tRNA molecules during protein synthesis. It can be found under the brand name Trobicin.

Spectinomycin can be retained and analyzed using the Obelisc R stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a Ammonium Acetate buffer. Detection is performed using CAD.

Condition
Column Obelisc R, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN -30%,
Buffer Ammonium Acetate pH 3.5 – 50 mM
Flow Rate 1.0 ml/min
Detection CAD

 

Description
Class of Compounds
  Antibiotics, Drug
Analyzing Compounds Spectinomycin

 

Application Column

Obelisc R

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Spectinomycin
Application Detection:
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.